Established in 1964, Lake Woodruff National Wildlife Refuge is comprised of 21,574 acres in central Florida along the St. Johns River (Florida's longest river) near DeLeon Springs. The Refuge has:
- 11,100 acres of freshwater marsh
- 7,200 acres of hardwood swamps
- 2,400 acres of uplands
- More than 1,000 acres of lakes, streams, and canals
The Refuge's geographical location straddles an overlapping zone between the subtropical and temperate climates. This overlap allows for a high diversity of species to be found on the Refuge. Additionally, it is uniquely positioned to support a wide variety of resident and migratory species.